RefNote: Screening of metagenomic imine reductases

Screening and characterization of a diverse panel of metagenomic imine reductases for biocatalytic reductive amination
https://www.nature.com/articles/s41557-020-00606-w

Background

Discovery Approach

of novel biocatalysis
Building Block

• direct evolution
• in silico screening / computational design
• explore existing sequence space
• exploiting cofactor versatility

Current Bottle Neck: HTS

• In silico: low in vitro “yield” (20/>500)
• expensive reactivity characterization: MS, microfluidic methods(fluorescence, absorbance)